The structure reveals the basis for proton transport, autoinhibition, and hexamer formation. 1A) and then extended into a hexameric model by symmetry-assisted placement of five additional copies into the hexamer map ( Fig. An atomic model was built into the monomer map ( Fig. An improved map at 3.21-Å resolution for the Pma1 monomer was derived from the same data by symmetry expansion of the hexameric particles followed by focused 3D classification and refinement using a monomer mask (table S1 and fig. We collected single-particle cryo-EM data of the hexameric form of Pma1 from Neurospora crassa plasma membrane in its autoinhibited state and obtained a hexamer map at 3.28-Å resolution.
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